ABSTRACT
The aim of this research is to investigate the preparation method of valve membrane in transcatheter bio- prosthetic valve, and to study the effect of chemical modification and cutting technology to tensile property and suture force property of valve membrane. We carried out a series of processes to perform the tests, such as firstly to test the crosslinking degree of valve membrane using ninhydrin method, then to test the tensile property and suture force property by using Instron's biomechanicAl testing equipment, and then to observe the collagen fiber orientation in valve membrane using Instron's biomechanical testing equipment and using field emission scanning electron microscopy. The study indicated that after the chemical modification, the crosslinking degree, tensile strength and suture force strength increasing rate of valve membrane were 93.78% ± 3. 2%, (8.24 ± 0.79) MPa, 102%, respectively. The valve membrane had a better biomechanical property and would be expected to become valve membrane in transcatheter bioprosthesis valve.
Subject(s)
Bioprosthesis , Collagen , Chemistry , Heart Valve Prosthesis , Microscopy, Electron, Scanning , Tensile StrengthABSTRACT
Biologic scaffold materials composed of extracellular matrix (ECM) are typically obtained in processes that involve decellularization of tissues or organs. Decellularized tissues and organs have been successfully used in a variety of tissue engineering/regenerative medicine applications. Preservation of the complex composition and three-dimensional ultrastructure of the ECM is highly desirable but it is recognized that all methods of decellularization result in disruption of the structure and potential loss of composition. The efficiency of cell removal from a tissue is dependent on the origin of the tissue and the physical, chemical, and enzymatic methods that are used. Each of these treatments affects the biochemical composition, tissue ultrastructure, and mechanical behavior of the remaining ECM scaffold, and all of the treatment methods affect the host response to the material as well. Tissue decellularization with preservation of ECM integrity and bioactivity can be optimized by making correct decisions regarding the agents and techniques utilized during processing. In this paper, the most commonly used decellularization methods are described, and consideration given to the effects of these methods upon the biologic scaffold material and recently described antigen removal strategy are presented.
Subject(s)
Animals , Humans , Acellular Dermis , Cell Death , Cell Separation , Extracellular Matrix , Chemistry , Tissue Engineering , Methods , Tissue ScaffoldsABSTRACT
Objective To investigate the effect of aspirin on platelet biochemical index in patients with cardio-cerebrovascular diseases and the influence of Naoxintong(Brain-heart unobstruction)Capsule.Methods The blood samples of 215 patients taking aspirin 100 mg per day for 7 days were collected for determination of platelet aggregation(PAG),P-selection level and TXB2 level by using arachidonic acid(AA)and adenosine diphosphate(ADP).Then they were divided randomly into aspirin group 1(n=72),still taking aspirin 100mg per day,aspirin group 2(n=70),taking aspirin 300mg per day,and Naoxintong group(n=73),taking aspirin 100mg per day and Naoxintong Capsule.One month later,their PAG,P-selection and TXB2 levels were re-examined.Results The plasma P-selection and TXB2 were in a positive correlation with AA-and ADP-induced PAG(P